澳洲CS作业代写 纳米孔的电荷和结构

“使生物孔适合DNA测序的结构性质是一个收缩位点,在这个位点上通过的链对电流的影响最大”。通道的冷态很大程度上决定了有多少碱基使电势发生变化。在特定时间同时“读取”的碱基数。读数应该是低的,以便为每个不同的碱基组合识别电流,而高的读数应该是允许某些后续碱基组合之间的重叠。这就形成了一种优势,同时通过基ecalling允许baes尽可能多地读取数据。为了开始测序,DNA链首先需要向孔的一侧移动,这也被称为顺方。在这个孔里,电场被捕获,它穿过孔,从另一个叫做反式的孔里出来。这里考虑了两种力量。电泳力是由反式上的正电场引起的,正电场吸引带负电荷的DNA并将其拉入。当负离子离开顺时针时,正离子向相反的方向移动。DNA链通过在孔的顺式入口周围形成正电荷带而得到加强。电渗透力是由离子流和净水通过孔洞引起的,并受链转移的影响。

澳洲CS作业代写 纳米孔的电荷和结构

“The structural property which makes the biological pore suitable for DNA sequencing is a constriction site at which the passing strand exerts the most influence on the electrical current”. The leng of the passage mostly determines how many bases influes the electric potential. The number of bases that is “read” concurrently at a particular time. Thenumber of reads should be low to allow identification of electric current for each different combinations of bases and high to allow the overlap between some subsequent combination of bases. This develops advantage while basecalling to allow baes to read as many times it is possible.To initiate the sequencing, the DNA strand first needs to move towards one side of the pore which is also named as cis-side. At this pore the electric field is captured and it is threaded through the pore and comes out at the other pore called trans-side.Here two forces are taken into consideration. Electrophoretic force is induced by positive electric field which is appied at the trans-side and attracts the negatively charged DNA and drags it in. The positive particles move to the opposite direction as the negative particles leave the cis-side. The DNA strands gets strengthened by the formation of positively charged zone around the cis entrance of the pore. The electro-osmotic flow force is induced by ion flow and net water through the pore which is influenced by strand translocation.

source : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5770995/

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